| Component | Volume (μL) | Final concentration |
|---|---|---|
| Genomic DNA in Nuclease-free water from Step 6A(x) | 41.5 | |
| NEBuffer 2 (10×) | 5.0 | 1× |
| S-adenosylmethionine (32 mM) | 1.0 | 0.64 mM |
| M.SssI methylase (20 U μL−1) | 2.0 | 0.8 U μL−1 |
| Total | 50 |
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