Component | Volume (μL) | Final concentration |
---|---|---|
Genomic DNA in Nuclease-free water from Step 6A(x) | 41.5 | |
NEBuffer 2 (10×) | 5.0 | 1× |
S-adenosylmethionine (32 mM) | 1.0 | 0.64 mM |
M.SssI methylase (20 U μL−1) | 2.0 | 0.8 U μL−1 |
Total | 50 |
Component | Volume (μL) | Final concentration |
---|---|---|
Genomic DNA in Nuclease-free water from Step 6A(x) | 41.5 | |
NEBuffer 2 (10×) | 5.0 | 1× |
S-adenosylmethionine (32 mM) | 1.0 | 0.64 mM |
M.SssI methylase (20 U μL−1) | 2.0 | 0.8 U μL−1 |
Total | 50 |