Capture probe maleimide plates |
5′-gtcgtgactgggaaaacttttttttttttttt-C6 thiol-3′ |
Reporter probe maleimide plates |
5′-HRP-actggccgtcgttttaca-3′ |
Capture probe test line |
5′- gtcgtgactgggaaaacttttttttttttttt-Biotin-TEG-3′ |
Capture probe control line |
5′-tgtaaaacgacggccagtttttttttttttttt-Biotin-TEG-3′ |
Reporter probe lateral flow (DNA 2) |
5′-actggccgtcgttttacattttttttttttttt-C6 thiol-3′ |
Reporter probe lateral flow (DNA 1) |
5′-actggccgtcgttttaca-C6 thiol-3′ |
Duplex DNA |
5′agctccagaagataaattacaggggccggggtggctcaggcaaggggttgacctgt 3′tcgaggtcttctatttaatgtccccggccccaccgagtccgttccccaactggaca cgtagggattgttttaacaactaggatactatgacccc-3′ gcatccctaacaaaattgttgatcctatgatactgggg-5′ |
Forward primer |
5′-gttttcccagtcacgac-C3-agctccagaagataaattacagg-3′ |
Reverse primer |
5′-tgtaaaacgacggccagt-C3-ggggtcatagtatcctagttg-3′ |