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. Author manuscript; available in PMC: 2017 Sep 13.
Published in final edited form as: Stem Cell Res. 2016 Sep 13;17(2):401–405. doi: 10.1016/j.scr.2016.09.004

Figure 1.

Figure 1

Characterization of H1.CD43/CD144DR dual reporter H1 hESCs. (A) Schematic of the constructs used for targeting of CD43-eGFP reporter into AAVS locus and VEC-tdTomato reporter by PiggyBac system. (B) Southern blot analysis of ApaL1 or EcoRV digested genomic DNA of CD43-eGFP cell. Asterisk, wild type; filled triangle, targeted; arrow, off-targeting. (C) Flow analysis of intracellular staining. Expression of transcriptional factors, OCT4, SOX2, and NANOG, by dual reporter and wild type of hESCs. (D) Differentiation of dual reporter cells to three germ layers. Expression of three germ layers markers AFP (endoderm), PAX6 (ectoderm), ETV2 and T (mesoderm), and GAPDH (internal control) in day 0, day 14 and 21 EBs of dual reporter H1.CD43/CD144DR (DR) cell line and wild type of H1 hESCs analyzed by RT-PCR. (E) Normal Karyotype (46, XY) of generated DR hESC line.