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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: J Immunol. 2016 Oct 26;197(11):4371–4381. doi: 10.4049/jimmunol.1601371

Fig. 6. BATF is not required for Th1 differentiation or function.

Fig. 6

(A) Th1 cultured CD4+ T cells, were stimulated with PMA/Ionomycin for 5 hours on the day of harvest. Representative contour plots of total CD4+ T cells from wild-type- and Batf−/−- IfngGreat mice with gates depicting the proportion of IFNg transcript (YFP). Histogram overlays and bar graphs depict the relative levels and frequency of IFNg transcript (YFP) and protein production. (B) Mice were exposed to N. brasiliensis and the lung (left lobe) was assessed 8 days later. Representative contour plots of isolated CD4+ T cells. Gate represents the percentage of IFNg transcript (YFP+) producing CD4+ T cells. Histogram overlay and bar graph depict the levels of IFNg transcript (YFP+) among indicated CD4+ T cell populations and the total number of IFNg transcript (YFP) producing cells, respectively. Ns: P>0.235 (two-tailed t-test). Data are representative of 3–4 (A) and cumulative of 2 (B) independent experiments. (A) n=2–3 replicate wells per group; (B) n=4–5 per group. Data are represented as mean +/− SD.