Table 1.
Tissue weight and body composition at week 7 before APN treatment, and at week 14 after APN treatment.
| Rag−/− |
|||||
|---|---|---|---|---|---|
| LFD |
HFD |
p-overall | |||
| (n=7) | PL (n=8) |
APN (n=6) |
|||
| Variable | week | Mean ± SE | Mean ± SE | Mean ± SE | |
| Fat Mass (g)** | 7 | 0.89 ± 0.11 b | 5.25 ± 0.72 a | 5.23 ± 0.37 a | 0.006 |
| 14 | 1.55 ± 0.24 | 6.01 ± 0.95 | 3.82 ± 0.15 | 0.24 | |
| change | 0.72 ± 0.21 a | 1.14 ± 0.64 a | −1.41 ± 0.53 b | 0.005 | |
| Fat Mass (%)** | 7 | 3.48 ± 0.41 b | 18.05 ± 2.01 a | 18.00 ± 1.21 a | 0.001 |
| 14 | 5.78 ± 0.81 | 17.93 ± 2.20 | 12.34 ± 0.34 | 0.09 | |
| change | 2.39 ± 0.71 a | 0.97 ± 1.50 a | −5.55 ± 1.40 b | 0.0001 | |
| Lean Mass (g)** | 7 | 18.39 ± 0.46 a | 17.86 ± 0.32 b | 18.55 ± 0.23 a | 0.001 |
| 14 | 19.22 ± 0.51 | 19.86 ± 0.37 | 20.28 ± 0.44 | 0.15 | |
| change | 0.83 ± 0.09 b | 2.00 ± 0.19 a | 1.73 ± 0.30 a | 0.03 | |
| Lean Mass (%)** | 7 | 73.55 ± 0.48 a | 62.28 ± 1.05 b | 64.51 ± 1.25 b | 0.0003 |
| 14 | 71.74 ± 0.49 a | 60.89 ± 1.69 c | 65.62 ± 0.73 b | 0.03 | |
| change | −1.81 ± 0.61 b | −1.40 ± 1.16 b | 1.10 ± 1.65 a | 0.0004 | |
| Body Weight (g) | 14 | 27.4 ± 0.7 b | 31.8 ± 0.9 a | 29.6 ± 0.8 ab | 0.005 |
| Heart (g) | 14 | 0.12 ± 0.01 | 0.13 ± 0.01 | 0.13 ± 0.00 | 0.99 |
| (%) | 14 | 0.44 ± 0.02 | 0.41 ± 0.01 | 0.43 ± 0.02 | 0.98 |
| Spleen (g) | 14 | 0.02 ± 0.00 | 0.03 ± 0.00 | 0.05 ± 0.01 | 0.08 |
| (%) | 14 | 0.09 ± 0.01 | 0.09 ± 0.01 | 0.17 ± 0.05 | 0.07 |
| Pancreas (g) | 14 | 0.39 ± 0.10 | 0.27 ± 0.04 | 0.24 ± 0.01 | 0.48 |
| (%) | 14 | 1.03 ± 0.07 | 0.86 ± 0.13 | 0.79 ± 0.03 | 0.42 |
| Liver (g) | 14 | 1.04 ± 0.04 | 1.35 ± 0.06 | 1.14 ± 0.02 | 0.06 |
| (%) | 14 | 3.79 ± 0.12 | 4.24 ± 0.11 | 3.86 ± 0.11 | 0.06 |
| Kidney (g) | 14 | 1.21 ± 0.04 | 1.14 ± 0.04 | 1.26 ± 0.05 | 0.22 |
| (%) | 14 | 0.33 ± 0.01 | 0.36 ± 0.01 | 0.37 ± 0.01 | 0.21 |
| Intestine | |||||
| Duodenum (g) | 14 | 0.24 ± 0.01 | 0.23 ± 0.02 | 0.20 ± 0.03 | 0.49 |
| (%) | 14 | 0.87 ± 0.05 | 0.73 ± 0.07 | 0.68 ± 0.11 | 0.43 |
| Jejunum (g) | 14 | 0.65 ± 0.06 | 0.59 ± 0.03 | 0.52 ± 0.03 | 0.08 |
| (%) | 14 | 2.36 ± 0.20 | 1.85 ± 0.11 | 1.76 ± 0.10 | 0.08 |
| Ileum (g) | 14 | 0.19 ± 0.02 | 0.15 ± 0.01 | 0.14 ± 0.01 | 0.19 |
| (%) | 14 | 0.71 ± 0.07 | 0.48 ± 0.04 | 0.50 ± 0.05 | 0.17 |
| Colon (g) | 14 | 0.17 ± 0.01 a | 0.12 ± 0.01 b | 0.12 ± 0.01 b | 0.001 |
| (%) | 14 | 0.62 ± 0.03 a | 0.39 ± 0.02 b | 0.41 ± 0.03 b | 0.001 |
| Adipose Tissue | |||||
| Epididymal (g) | 14 | 0.49 ± 0.06 | 1.30 ± 0.16 | 0.94 ± 0.07 | 0.06 |
| (%) | 14 | 1.80 ± 0.28 b | 4.05 ± 0.37 a | 3.17 ± 0.19 a | 0.02 |
| Perirenal (g) | 14 | 0.09 ± 0.01 | 0.37 ± 0.07 | 0.21 ± 0.02 | 0.18 |
| (%) | 14 | 0.34 ± 0.05 c | 1.15 ± 0.16 a | 0.71 ± 0.04 b | 0.05 |
| Mesenteric (g) | 14 | 0.25 ± 0.06 | 0.38 ± 0.06 | 0.25 ± 0.02 | 0.58 |
| (%) | 14 | 0.91 ± 0.20 | 1.18 ± 0.16 | 0.85 ± 0.07 | 0.61 |
| Subcutaneous (g) | 14 | 0.25 ± 0.02 b | 0.72 ± 0.10 a | 0.39 ± 0.02 b | 0.03 |
| (%) | 14 | 0.90 ± 0.07 b | 2.24 ± 0.23 a | 1.34 ± 0.08 b | 0.01 |
| Brown (g) | 14 | 0.08 ± 0.01 | 0.13 ± 0.01 | 0.09 ± 0.01 | 0.32 |
| (%) | 14 | 0.28 ± 0.05 | 0.42 ± 0.03 | 0.31 ± 0.03 | 0.33 |
Abbreviations: LFD, low fat diet; HFD, high fat diet; PL, placebo; APN, adiponectin; The p-overall refers to the p value after performing a one-way analysis of variance for the investigated parameter. For the parameters showing a p-overall <0.05, a post-hoc analysis between the different groups was performed (Means with different letters a,b,c are significantly different, p <0.05). P-overall values were adjusted for body weight.
**
The values measured by MRI.