Table 2.
AgRP, POMC, NPY and MC4R mRNA expression in hypothalamus, UCP mRNA expression in muscle and brown adipose tissue
| LFD (n=7) |
HFD-PL (n=8) |
HFD-APN (n=6) |
p-overall | |
|---|---|---|---|---|
| Variable | Mean ± SE | Mean ± SE | Mean ± SE | |
| Hypothalamus | ||||
| AgRP | 0.55 ± 0.06 a | 0.28 ± 0.04 b | 0.46 ± 0.07 a | 0.0094 |
| POMC | 0.68 ± 0.04 | 0.83 ± 0.11 | 1.09 ± 0.15 | 0.15 |
| NPY | 0.85 ± 0.04 | 0.75 ± 0.08 | 0.92 ± 0.09 | 0.35 |
| MC4R | 0.73 ± 0.07 | 0.78 ± 0.09 | 0.81 ± 0.09 | 0.88 |
| Muscle | ||||
| UCP1 | 1.01 ± 0.50 | 2.15 ± 1.45 | 1.02 ± 0.44 | 0.85 |
| UCP2 | 0.67 ± 0.11 | 0.71 ± 0.07 | 0.97 ± 0.25 | 0.43 |
| UCP3 | 1.36 ± 0.08 | 1.16 ± 0.12 | 1.39 ± 0.16 | 0.15 |
| Brown Adipose Tissue | ||||
| UCP1 | 0.85 ± 0.05 | 0.81 ± 0.05 | 0.86 ± 0.05 | 0.89 |
| UCP2 | 0.97 ± 0.15 | 0.89 ± 0.02 | 0.94 ± 0.03 | 0.82 |
| UCP3 | 1.05 ± 0.04 | 0.86 ± 0.05 | 0.94 ± 0.05 | 0.08 |
Abbreviations: LFD, low fat diet; HFD, high fat diet; PL, placebo; AP, adiponectin; UCP, Uncoupling protein; AgRP, agouti-related peptide; POMC, proopiomelanocortin; NPY, neuropeptide Y; MC4R, melanocortin 4 receptor; The p-overall refers to the p value after performing a one-way analysis of variance for the investigated parameter. For the parameters showing a p-overall <0.05, a post-hoc analysis between the different groups was performed (Means with different letters a,b,c are significantly different, p <0.05). P-overall values were adjusted for body weight.