Figure 2. In A20^cko and A20/Myd88^cko mice, intestinal DCs are phenotypically mature and expand pathological mucosal T cells that cause small intestine inflammation.

(a) Representative flow cytometry gating strategy in A20^wt mice to identify the three bona fide DC populations of SI-LP. (b) Expression of A20 mRNA by the indicated SI-LP DC subset or macrophages from wild-type mice, relative to hprt. (c-f) Cell surface expression of maturation markers CD80 (c), CD86 (d) CD40 (e) and total cell number (f) of SI-LP DCs and macrophages. Cellular expression of maturation markers is represented as mean fluorescence intensity (MFI) relative to that of the same population in wild-type (WT) mice. (g) Cell number of SI-LP CD4⁺ T cells, IFNγ⁺, IL-17⁺, IFNγ⁺IL-17⁺ and Foxp3⁺CD4⁺ T cells from mice of the indicated genotype. Data in (c-g) was combined from at least 3 independent experiments with mice between 10-14 weeks of age, including at least one mouse of each genotype per experiment. Each dot represents one mouse. (h) Organ weights of small intestine from Rag1^-/- mice of the indicated genotypes (n = 9-11 mice of each genotype, aged 12-14 weeks). Error bars represent mean ± SEM, *, P <0.05, **, P <0.01, ****, P <0.0001 (unpaired student's t-test). See also Figure S2.