Skip to main content
. Author manuscript; available in PMC: 2017 Oct 20.
Published in final edited form as: Mol Cell. 2016 Oct 6;64(2):376–387. doi: 10.1016/j.molcel.2016.09.005

Figure 3. Lesion-Dependent Motions in Damage Recognition by Rad4-Rad23.

Figure 3

(A) Single frame of quantum dot-labeled Rad4-Rad23 assembled in an array on Fl-dT-containing DNA. See also Movie S4.

(B) Kymograph of Rad4-Rad23 particles assembled on Fl-dT array shown in (A).

(C) Single frame of quantum dot-labeled Rad4-Rad23 assembled in an array on CPD-containing DNA. Rad4-Rad23 particles are indicated by white arrows. See also Movie S5.

(D) Kymograph of Rad4-Rad23 particles assembled on CPD array shown in (C).

(E) Distributions of motion types of WT Rad4-Rad23 observed on DNA damage arrays show lesion-dependent behavior (Fl-dT – green, CPD – orange, undamaged DNA – purple, UV-irradiated λ-DNA – gray). See also Figure S2 and S3.

(F) and (G) Distributions of motion types of WT, Δβ-hairpin3, and ΔBHD3 observed on DNA damage arrays containing sites of Fl-dT (WT – red, Δβ-hairpin3 – green, ΔBHD3 – blue), and CPD (WT – pink, Δβ-hairpin3 – mint, ΔBHD3 – lavender), respectively. WT data reproduced from Figure 3E.