(A) Distributions of observed motion types from Rad4 WT and mutants.
(B – D) Anomalous diffusion exponent (α) vs. diffusion coefficient (log10D) plotted for random (filled circles) and constrained (empty circles) particles of WT (B), Δβ-hairpin3 (C), and ΔHD3 (D). See also Figure S4.
(E) Dissociating particles as fractions of total particles observed increase with larger deletions in Rad4 BHD3 sequence.
(F) Cumulative residence time distribution (CRTD) plot of lifetimes of Rad4 WT and mutants that dissociated during observation. See also Figure S5.