Figure 1.

Developing an in vitro model of in vivo haemorrhagic shock. (A) Huh7 cells were exposed to hypoxia, hypercapnia and/or hypothermia for 4 h as indicated and analysed for pGSK3β‐Ser⁹ levels and normalized to Nx. (B) Huh7 cells were exposed to normoxic conditions (37°C, 5% CO₂; Nx), or combined hypoxia, hypercapnia and hypothermia (HxHcHp), treated with VPA (0.75 mM) as indicated, analysed for pGSK3β‐Ser⁹ levels and normalized to Nx. Data were quantified from at least triplicate experiments with technical triplicates (n > 9) ± SEM. Data were analysed using one‐way ANOVA and post hoc Tukey test.