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. 2015 Oct 22;172(22):5306–5317. doi: 10.1111/bph.13320

Figure 2.

Figure 2

Defining the haemorrhagic shock signalling pathway. Huh7 cells were incubated for 4 h in the presence of hypoxia (2% O2), hypercapnia (10% CO2) and hypothermia (32°C) (HxHcHp), and treated with VPA as indicated. All data are shown as mean ± SEM normalized to normoxic conditions (Nx). Phosphorylation levels are presented as percentage of untreated control and corrected for loading with loading control indicated. (A) An overview of PI3K signalling pathway regulation by haemorrhagic shock signalling (stress response) and VPA treatment (VPA response). (B) Protein extract was analysed for PTEN phosphorylation levels at Ser380/Thr382/383. (C) Protein extract was analysed for Akt phosphorylation levels at Ser473. (D) Protein extract was analysed for total β‐catenin levels. (E) Protein extract was analysed for histone (H)2/3 and H4 acetylation using acetylated lysine antibody. Data were quantified from at least triplicate experiments with technical triplicates (n ≥ 9) ± SEM. Data were analysed using one‐way ANOVA and post hoc Tukey test (B, C and D) or using two‐way ANOVA and post hoc Bonferroni tests (E).