Table 1.
List of PCR primers for detection various gene expression or cloning of cDNAs.
| Rat gene | Primers | Product size (bp) |
|---|---|---|
| Rt1bα | F, 5′-AGAACAGAGATGCCGCTCAGCAGAGC-3′ R, 5′-TTCCCAGGATGGTACTCAAAGGGGCC-3′ |
795 |
| Clip (Il) | F, 5′-TGTTCTTGTGAAGACAGAAGCCAGC-3′< >R, 5′-GTATCCAGCCTAGATTAAGGGTAGG-3′ |
315 |
| Tcrε | F, 5′-GGTACTGGTGGCAGGCCCAGAGG-3′ R, 5′-GTTCTAGGATGCGTGTTCACCAGG-3′ |
250 |
| Gapdh | F, 5′-CTTCACCACCATGGAGAAGGC-3′ R, 5′-GGCATGGACTGTGGTCATGAG-3′ |
238 |
| Tyrobp | F, 5′-GCTTCAGGGTCAGAGGCCAGAA-3′ R, 5′-TGGGGACAGAAACGGTACAAGG-3′ |
218 |
| Ly49s3 | F, 5′-GTCTGTATTTCAGTATGTCAGG-3′ R, 5′-ATTCTCCACATGCAAGCTCCTC-3′ |
236 |
| Ly49s4 | F, 5′-GTATGTATTTCAGTATGTCAGG-3′ R, 5′-TCACACAGCAGAATGTGACC-3′ |
135 |
| Ly49s5ORF | F, 5′-TCCCAAAATGAGTAAGCAGGATG-3′ R, 5′-ATCATCTCTCTATTCACACAGCAG-3′ |
854/741 |
| Ly49s6 | F, 5′-CTCCCAAAATGAGTAAGCAGG-3′ R, 5′-CATCTCTTTATTCACAGAGCAG-3′ |
614 |
| Ly49TM/IC | F, 5′-ACAGACTTCATACATCATTCCC-3′ R, 5′-AACAGCAACCAGCCGAAG-3′ |
199/208 |
| Gzma | F, 5′-CTGGGTGTCCTCTCTTACTACTGTC-3′ R, 5′-TTAAACAGCGCCCTTCGCAGTCTTC-3′ |
766 |
| Gzmb | F, 5′-TTTCCTAGAGGTGAAGAGAGCAAGG-3′ R, 5′-AGGGCAGATGGTTAGTCAGGAAATG-3′ |
860 |
| Gzmc ORF | F, 5′-GAGGATGCCACCAGTCCTGATACTC-3′ R, 5′-GCTATAGGGAAGATGGTTAGTCAGG-3′ |
800 |
| Pfr | F, 5′-CTCAAGCGAATACAAAGCTTGCGAGG-3′ R, 5′-TTGTTTTTACCACACAGCCCCACTGC-3′ |
859 |
| Rt1ce5 | F, 5′-CCTTCCACATTCAACCTTGCTG-3′ R, 5′-TGTAGAAGTGAGGAGTTGCAG-3′ |
103 |
| Rt1ce14 | F, 5′-CCTTCCAGAAGTGGGCATCTGT-3′ R, 5′-ATGGCCACAGCTCCAATGATGG-3′ |
211 |
| Rt1ce16 | F, 5′-GCAGTTGAATGGGGAGGACC-3′ R, 5′-ATCACAACAGCCACCACAGC-3′ |
286 |
| Faslg | F, 5′-AAAGACCACAAGGTCCAACA-3′ R, 5′-AGTCTCTAGCTTATCCCATGA-3′ |
341 |
| Lat | F, 5′-CCCACACAGAC GGCATCTCC-3′ R, 5′-CTGAGCATCTGGAGTGGCCC-3′ |
210 |
F, forward; R, reversed