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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: J Immunol. 2016 Oct 19;197(10):4053–4065. doi: 10.4049/jimmunol.1600323

FIGURE 1. Detection of extracellular NS1 derived from DENV-infected insect cells.

FIGURE 1

C6/36 (black bars), PscloneD (white bars) and BHK (grey bars) cells were infected with DENV at an MOI of 0.01, 0.1 or 1.0 for 2 days. The levels of secreted NS1 (A), cell viability (B), percentage of NS1- (C) and E- (D) expressing cells and DENV production (E) were determined by NS1-ELISA, flow cytometry analysis using propidium iodide exclusion, anti-NS1 mAb (clone 1F11) and anti-E mAb (clone 4G2) and FFA, respectively. The data are shown as mean ± standard deviation (SD) of three to four independent experiments. Asterisks denote levels of NS1 detected in supernatants that are statistically different (* P < 0.05). The correlation coefficients between secreted NS1 and DENV production in DENV-infected C6/36 (F), DENV-infected PscloneD (G) and DENV-infected BHK (H) were analyzed by linear regression. NS1 in 50-fold concentrated supernatants of DENV-infected C6/36, AP-61 and TRA-284 cells was analyzed by Western blotting using anti-DENV NS1 mAb clone 1F11 (I).