Figure 3.

YTHDF Proteins Relocalize to Lipid Droplets during HCV Infection

(A) Confocal micrographs of HCV-infected or uninfected Huh7 cells (48 hpi) immunostained with antibodies to YTHDF (green) and HCV Core (red) proteins. Lipid droplets (gray) and nuclei (blue) were labeled with BODIPY and DAPI, respectively. Zoom panels are derived from the white box in the merge panels. Scale bar, 10 μm.

(B) Enrichment of YTHDF proteins around lipid droplets was quantified using ImageJ from more than ten cells analyzed and graphed in box-and-whisker plots, representing the minimum, first quartile, median, third quartile, and maximum. ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001 by unpaired Student’s t test.

See also Figure S3.