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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: J Immunol. 2016 Oct 28;197(11):4344–4350. doi: 10.4049/jimmunol.1601070

Figure 4. Lin28b regulates TGFβ signaling in fetal T cells through regulation of let-7.

Figure 4

a–c. Flow cytometry analysis of staining for TGF-βRI, TGF-βRIII, and SMAD2 on siCtrl- and siLin28b-treated fetal CD4+ T cells. Graphs show MFI of TGF-βRI, TGF-βRIII, and SMAD2 on siCtrl- and siLin28b-treated CD4+ T cells. d. Flow cytometry analysis of staining for intracellular phosphorylated SMAD2/SMAD3 on siCtrl- and siLin28b-treated CD4+ T cells in the presence of 2 ng TGF-β. Bar graph shows phosphorylated SMAD2/SMAD3 MFI within the CD4+ population. e. qRT-PCR analysis of TGF-βRI, TGF-βRIII, and SMAD2 mRNA expression in Jurkat cells transfected with control or let-7g miRNA. The data are representative of results from 8 fetal and 8 adult individuals in 3 independent experiments. Statistics refer to Wilcoxon test. *** p<.001, ** p<.01, * p<.05