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. Author manuscript; available in PMC: 2016 Nov 25.
Published in final edited form as: Cell Rep. 2016 Nov 1;17(6):1532–1545. doi: 10.1016/j.celrep.2016.10.019

Figure 4. SK1-I accumulates sphingolipids in enlarged late endosomes.

Figure 4

WT (A–D) or SphK1−/− (E–H) MEFs were treated with DMSO or 10 μM SK1-I for 4 h followed by subcellular fractionation. Protein expression was quantified by immunoblot and presented as percent enrichment in each fraction. Sphingolipids were quantified by LC-MS/MS and presented as pmol lipid per fraction. (A and E), Lamp1. (B and F), Ceramide. (C and G), Sphingomyelin. (D and H), Monohexosylceramide. See also Figure S5.