Figure 3. Formation of HAA derived DNA adducts and CYP1 activity in activated human T lymphocytes.

(A) CYP1 activity in PMA/Iono activated human T lymphocytes.. After 72 h of activation with PMA/Iono, the cells were treated with 0.1% DMSO (Ctrl) or with 1 μM of 3-MC for 24 h and CYP1 activity was measured. Data are expressed as the mean ± SD of eight independent experiments. (Student’s t-test, * P<0.05; **P<0.01, ***P<0.005). ND: not detectable (B) Kinetics of 4-ABP and HAA DNA adduct formation in PMA/Iono activated human T lymphocytes. After 72 h of activation with PMA/Iono, the cells were treated with PhIP, MeIQx, AαC or 4-ABP (10 μM) for 3, 8 or 24 h, and the DNA adducts were measured by UPLC/MS³. Data are representative of five donors; the DNA adduct level was measured in duplicate for each donor. Data are expressed as Mean ± SD. (C) CYP1 activity in CD3/CD28 activated human T lymphocytes. CD3+ T cells were either unstimulated (UNS) or activated with anti-CD3 and anti-CD28 Antibodies for 72 h and CYP1 activity was measured after 24 h of treatment with 0.1% DMSO (Ctrl) or 3-MC (1 μM). Data are expressed as the mean ± SD of four independent experiments. (Student’s t-test, * P<0.05; **P<0.01, ***P<0.005). (D) Levels of DNA adducts derived from 4-ABP, AαC and PhIP in CD3/CD28 activated human T lymphocytes. CD3+ T cells were activated with anti-CD3 and anti-CD28 antibody for 72 h, and the level of DNA adducts were measured after 24 h of treatment with PhIP, AαC or 4-ABP (10 μM). DNA adducts levels were measured in duplicate for each donor and are expressed as the mean ± SD. The data are representative of four independent donors. ND: not detectable