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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Environ Mol Mutagen. 2016 Nov 1;57(9):656–667. doi: 10.1002/em.22059

Figure 5. Regulation of CYP1A1 and CYP1B1 mRNA expression in activated human T lymphocytes by HAA.

Figure 5

Regulation of (A) CYP1A1 and (B) CYP1B1 mRNA expression by HAA in activated human T lymphocytes. PMA/Iono activated human T lymphocytes were treated for 24 h with 0.1% DMSO (Ctrl), HAA (10 μM) or 3-MC (1 μM) and CYP1A1 and CYP1B1 mRNA expression were analyzed by qRT-PCR. The levels of expression were normalized with 18s mRNA. Data are representative of five independent experiments and expressed as the mean ± SD. (ANOVA followed by Dunnett’s multi-range test, * P<0.05; **P<0.01, ***P<0.005 versus Ctrl). Effect of Actinomycin D (Act D) and Cycloheximide (CHX) on AαC induced (C) CYP1A1 and (D) CYP1B1 mRNA expression in activated human T lymphocytes. PMA/Iono activated human T lymphocytes were treated with PhiP, MeIQx and AαC (10 μM) or 3-MC (1 μM) for 16 h with or without Act D or CHX and the levels of CYP1A1 and CYP1B1 mRNA expression were analyzed by qRT-PCR. The data were normalized with 18s mRNA. Data are representative of three independent experiments and expressed as the mean ± SD. (ANOVA followed by Tukey’s multi-range test, * P<0.05; **P<0.01, ***P<0.005 versus Ctrl). Each treatment from Act D and CHX group has been compared to its corresponding treatment in the control group.