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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Environ Mol Mutagen. 2016 Nov 1;57(9):656–667. doi: 10.1002/em.22059

Figure 6. Effect of CH-223191 and α-Naphtoflavone (α-NF), two AhR antagonists on PhIP and AαC derived DNA adducts formation and CYP1 activity in activated human T lymphocytes.

Figure 6

Effect of CH-223191 and α-NF on AαC induced (A) CYP1A1 and (B) CYP1B1 mRNA expression in activated human T lymphocytes. PMA/Iono activated human T lymphocytes were treated for 1 hour with 0.1% DMSO (Ctrl), CH-223191 (5 μM) or α-NF (5 μM), followed by 24 h of treatment with 0.1% DMSO (Ctrl), HAA (10 μM) or 3-MC (1 μM). The levels of CYP1A1 and CYP1B1 mRNA expression were analyzed by qRT-PCR and normalized with 18s mRNA. Data are representative of three independent experiments and expressed as the mean ± SD. (ANOVA followed by Dunnett’s multirange test, * P<0.05; **P<0.01, ***P<0.005, versus Ctrl). Each treatment from CH-223191 and α-NF group has been compared to its corresponding treatment in the control group. (C) Effect of CH-223191 and α-NF on DNA adducts derived from AαC and PhIP formation in activated human T lymphocytes. PMA/Iono activated human T lymphocytes were pre-treated for 1 hour with 0.1 % DMSO (Ctrl), CH-223191 (5 μM) or α-NF (5 μM) followed by 24 h of treatment with 0.1% DMSO (Ctrl) or HAA (10 μM). The DNA adducts were measured by UPLC/MS3. DNA adducts were measured in duplicate for each donor and expressed as the mean ± SD. (ANOVA followed by Tukey’s multiple comparison test * P<0.05; **P<0.01, ***P<0.005, versus Ctrl). ND: not detectable. (D) Effect of CH-223191 and α-NF on CYP1 activity in activated human T lymphocytes. CYP1 activity was measured in PMA/Iono activated human T lymphocytes treated for 1 hour with 0.1 % DMSO (Ctrl), CH-223191 (5 μM) or α-NF (5 μM) followed by 24 h of treatment with 0.1% DMSO (Ctrl), PhIP (10 μM), AαC (10 μM) or 3-MC (1 μM). Data are representative of three independent experiments and expressed as the mean ± SD. (ANOVA followed by Tukey’s multiple comparison test, * P<0.05; **P<0.01, ***P<0.005 versus Ctrl). ND: not detectable.