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. Author manuscript; available in PMC: 2016 Nov 25.
Published in final edited form as: Reproduction. 2006 May;131(5):905–915. doi: 10.1530/rep.1.01046

Figure 5.

Figure 5

Detection of multiple SSB-1-related species in transfected cells. (A) CHO cells that were not transfected, transfected with a plasmid encoding SSB-4 or transfected with a plasmid encoding SSB-1 were harvested 24 or 48 h post-transfection and imunoblotted using affinity-purified anti-SSB-1. The 40 kDa band in all lanes is probably unrelated to SSB-1 (see text). SSB-1-transfected cells display a 31 kDa band 24 h after transfection and a weaker 36 kDa band that is more intense 48 h after transfection. (B) HeLa cells were not transfected or were transfected with the construct encoding SSB-1 and blotted using affinity-purified anti-SSB-1. In addition to the 31 kDa band, several higher molecular mass immunoreactive species are present.