Stress conditioning increases cardiomyocyte hypertrophy. A, MYH7 positive area was measured to determine cardiomyocyte size from constructs in the different conditioning regimes. Cardiomyocyte size increased ~50% from NS vs. SS constructs, with no further increase in the ES group.(NS to SS, NS to SE, p<0.0001) B, C, and D, show MYH7 positive cells in constructs from NS, SS, and SE, respectively. n=16 for NS, n=14 for SS, and n=14 for SE. Hematoxylin counterstain denotes nuclei. The scale bar is 50 μm.