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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: J Neurochem. 2016 Oct 18;139(5):737–747. doi: 10.1111/jnc.13852

Figure 1.

Figure 1

Chronic morphine increased the association of MOR and Gsα with Cav-1. Membranes were obtained from opioid naïve (Nv) and chronic morphine (CM) treated (1 μM, 48 h) MOR-CHO. The Triton insoluble fraction was isolated, solubilized, and subjected to immunoprecipitation with anti-Cav-1 antibody as described in methods. Following SDS PAGE and electrotransfer of proteins, regions of the nitrocellulose membrane corresponding to MOR, Gsα, and Cav-1 were cut out and Western blotted with their corresponding antibodies. Quantification of Western signals corresponding to A: MOR (≈80 kDa) and B, Gsα (≈45 kDa) in Cav-1 ip was normalized to their corresponding directly IPed Cav-1 protein. The content of MOR or Gsα in the Cav-1 IP obtained from chronic morphine-treated MOR-CHO is expressed as a percent of their content in Cav-1 IP obtained from opioid naïve cells. Following chronic morphine, the association of MOR and Gsα with Cav-1 within caveolae/Cav-1 scaffolds is significantly increased. n=4–5; *indicates p<0.05.