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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: J Neurochem. 2016 Oct 18;139(5):737–747. doi: 10.1111/jnc.13852

Figure 3.

Figure 3

Chronic morphine augmented the association of MOR with Gsα. The Triton insoluble fraction obtained from membranes of opioid naïve (Nv) and chronic morphine (CM) treated MOR-CHO (1 μM, 48 h) was solubilized and subjected to immunoprecipitation using anti-Gsα antibodies. Co-IPed MOR was visualized by Western blotting. Quantification of band intensity was normalized to their corresponding directly IPed Gsα protein. The content of MOR in the Gsα IP obtained from chronic morphine-treated MOR-CHO is expressed as a percent of the MOR content of Gsα IP obtained from opioid naïve cells. Chronic morphine increased the association of MOR and Gsα within lipid raft compartments. n=4; *=p<0.01.