Skip to main content
. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Kidney Int. 2016 Aug 31;90(6):1262–1273. doi: 10.1016/j.kint.2016.06.037

Figure 2. Mesenchyme-specific deletion of Zeb2 using Six2-cre also leads to glomerulocystic kidney disease without tubular dilatation.

Figure 2

(a) Dilated glomerular Bowman’s space (asterisk) was observed in the Zeb2flox/flox;Six2-cre+ embryonic kidneys at E16.5 but not in littermate controls (H&E staining, 200x magnification). (b) Glomerular cysts (asterisk) in the subcapsular region of the kidney cortex at P12 in the Zeb2flox/flox;Six2-cre+ kidneys but not in the littermate controls (H&E staining, 200x magnification). (c) IHC staining shows the cyst (cy) in a P12 Zeb2flox/flox;Six2-cre+ kidney is negative for the proximal tubules marker Lotus Tetragonolobus Lectin (LTL). Upper panels - 100x magnification; lower panels - high magnification of boxed regions in the upper panels (n≥3 for each group). (d) IHC staining shows the cysts (cy) in a P12 Zeb2flox/flox;Six2-cre+ kidney is negative for the collecting duct and distal tubules marker Dolichos Biflorus Agglutinin (DBA). Upper panels - 100x magnification; lower panels - high magnification of boxed regions in the upper panels (n≥3 for each group). Abbreviations: g-glomerulus; cy-cyst.