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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Kidney Int. 2016 Aug 31;90(6):1262–1273. doi: 10.1016/j.kint.2016.06.037

Figure 4. Mesenchyme-specific deletion of Zeb2 with Six2-cre causes glomerulosclerosis, albuminuria and renal failure in adult mice.

Figure 4

(a) Masson Trichrome staining (MTS) shows minimal fibrosis (blue) in the kidney of a 7 weeks old Zeb2flox/flox;Six2-cre+ mouse. Upper panels - 100x magnification; lower panels - high magnification of boxed regions in the upper panels (n=3 for each group). (b) Periodic acid Schiff (PAS) staining shows glomerulosclerosis (arrow) in non-cystic glomeruli of a 7 weeks old Zeb2flox/flox;Six2-cre+ mouse kidney. Upper panels - 100x magnification; lower panels - high magnification of boxed regions in the upper panels (n=3 for each group). (c) Representative SDS-page gel with Coomassie blue staining shows albuminuria (arrow) in two Zeb2flox/flox;Six2-cre+ 5 weeks old mice but not in two littermate controls (n=5 mice in each group); alb - albumin. (d) Increased albumin-to-creatinine ratio (ACR) in 5 weeks old Zeb2flox/flox;Six2-cre+ mice compared to Zeb2+/+ littermate controls (n=4 mice in each group, *p < 0.05) (e) Significantly elevated BUN in 5 weeks old Zeb2flox/flox;Six2-cre+ mice compared to Zeb2+/+ littermate controls (n=3 mice in each group, **p-value< 0.001). Data are represented as means +/− standard deviation.