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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Kidney Int. 2016 Aug 31;90(6):1262–1273. doi: 10.1016/j.kint.2016.06.037

Figure 5. Congenital atubular glomeruli in Zeb2 mesenchyme-specific knockout mice.

Figure 5

(a) LTL staining shows many glomerular Bowman’s capsule and proximal tubule connections (arrows) in P12 Zeb2+/+ wild-type control mice but very few connections in Zeb2flox/flox;Six2-cre+ mutant mice. Upper panels - 100x magnification; lower panels - 400x magnification; g -glomerulus. (n=5 in each group); Scale bars are shown. (b) Percentage of LTL-positive glomeruli out of total glomeruli counted in sagittal kidney sections from five Zeb2 cKO and five Zeb2+/+ littermate controls (n=604 glomeruli in Zeb2 cKO and n=324 glomeruli in wild-type were counted, *p < 10−3). (c) Representative serial sections of P12 Zeb2+/+ wild-type kidneys (upper panels) and Zeb2flox/flox;Six2-cre+ cKO kidneys (lower panels) show the detection of glomerulotubular junctions (arrows) in the wild-type glomeruli but absence in many Zeb2 cKO glomeruli (* indicating the same glomerulus on serial sections).