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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Kidney Int. 2016 Aug 31;90(6):1262–1273. doi: 10.1016/j.kint.2016.06.037

Figure 6. Decreased early proximal tubular marker expression in Zeb2 mesenchyme-specific knockout mice.

Figure 6

(a) At postnatal day 8 (P8), TaqMan assays show decreased mRNA expression levels of proximal tubular markers (Vil1 and Hnf1a) but not podocyte markers (Nphs1 and Nphs2) in Zeb2flox/flox;Six2-cre+ kidneys when normalized to a collecting duct marker Ppp1r3c. Collecting duct marker Upk3a was used as a control (n= 3 in each group, mean relative quantification adjusted to Ppp1r3c, *p < 0.05). (b) At E14.5, TaqMan assays show decreased mRNA expression levels of proximal tubular mRNA markers (Vil1 and Hnf1a) in the cKO kidneys compared to wild-type kidneys. There are no differences for the metanephric mesenchyme markers (Pax2 and Wt1) and the collecting duct markers (Ppp1r3c and Upk3a) in cKO and control kidneys (mean relative quantification adjusted to Gapdh, n=3 in each group, *p <0.05). (c) Immunofluorescent staining shows decreased expression of VIL1 (villin-1) protein in E15.5 Zeb2flox/flox;Pax2-cre+ cKO proximal tubules (arrowhead) as compared to the proximal tubules in wild-type littermates (arrows). (d) Decreased expression of VIL1 (villin-1) protein in E16.5 Zeb2flox/flox;Six2-cre+ cKO proximal tubules (arrowhead) as compared to the proximal tubules in wild-type littermates (arrows). (e) The Zeb2 cKO embryos have smaller kidney length at E16.5 as compared to wild-type controls (n=7 for cKO kidneys and n=6 for wild-type kidneys, *p <10−3).