JNK phosphorylation in liver cytosolic fraction and mitochondrial p-JNK translocation after APAP treatment in C57BL/6J and C57BL/6N mice. Animals were treated with 200 mg/kg APAP or saline as control. At 2 h and 8 h after APAP, cytosolic and mitochondrial fractions were subjected to western blotting for phosphorylated JNK, total JNK, porin and beta-actin (A and B). Densitometry was performed on these blots and the pJNK-to-JNK ratio, the pJNK-to-beta actin ratio and pJNK-to-Porin ratio was calculated for the different time points of C57BL/6J and C57BL/6N mice Data represent mean ± SE of n=3 animals per group. *p< 0.05 (compared with controls, t=0). #p< 0.05 (compared with C57BL/6J mice treated with APAP).