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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Stem Cells. 2016 Sep 1;34(12):2916–2929. doi: 10.1002/stem.2471

Figure 4.

Figure 4

CMCs depleted of HDAC1 exhibit enhanced expression of stemness-, cardiac-, and endothelial lineage-specific markers. (A): Epifluorescence microscopy images depicting efficiency of CMC lentiviral transduction (PGK-GFP control vector). (B): The efficiency of HDAC knockdown in CMCs transduced with shHDAC1, shHDAC4, shHDAC7, or non-targeting shRNA control (NT shRNA) was evaluated via real-time qPCR (bar graphs; n=7) and Western analysis (insets; n=3). Values are mean (n=7) ± SEM. † P <0.005 (Relative to NT shRNA). (C-F): The influence of HDAC knockdown on stemness-, cardiac-, smooth muscle- or endothelial-lineage specific gene transcription, respectively, was assessed via real-time qPCR 72 h after lentiviral transduction. Values are mean (n=7) ± SEM. * P <0.05 (Relative to NT shRNA) and † P <0.005 (Relative to NT shRNA).