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. Author manuscript; available in PMC: 2018 Jan 5.
Published in final edited form as: Mol Cell Endocrinol. 2016 Sep 20;439:346–353. doi: 10.1016/j.mce.2016.09.021

Figure 4. GnRHa-mediated actin reorganization involves mTORC2.

Figure 4

LβT2 cells were grown on glass-bottom microwell dishes for 24 h. Cells were pretreated (30 min) with vehicle (0), RAPA (10nM), or PP242 (50μM) followed by a 15 min treatment of either vehicle (0) or GnRHa (10nM). Cells were fixed in 4% PFA and then stained with Alexa 488-conjugated phalloidin to label actin and DAPI to label nuclei. All cells were imaged under a 63X oil objective of a Zeiss LSM 710 confocal microscope.