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Morphine enhances P2X4R expression in microglia. Microglia were treated with morphine (100 μM, once daily) or together with LPS-RS (10 ng/ml, once daily) for 1, 2, or 3 days, lysed, and subjected to Western blot analysis. a Morphine induced time-dependent increasing of P2X4R expression. b P2X4R expression was inhibited with LPS-RS treatment for 3 days. (#p < 0.05, compared with saline; *p < 0.05, compared with morphine; n = 6)
