Fig. 2.
Time course patterns exhibited by c-Fos induction. a Time course of c-Fos induction after LIC, D + A, D + S, PTH: a maximum peak at 60 min and depletion at 120 min were observed in LIC, D + A and D + S. PTH induced a maximum induction at 30 min and depletion at 60 min. b A maximum peak at 60 min and depletion at 120 min were observed after GLP-1, with no significant differences compared to LIC; GLP-2 had similar increase as GLP-1 at 60 min, but no depletion was observed at 120 min, with higher levels that GLP-1 and LIC. c GLP1 combined with ATP 10−5 M induced a maximum peak at 60 min and depletion at 120 min; GLP-2 had an increase at 30 min, and depletion was observed at 60 min. Results are expressed as the average of fold change, n = 6, ±SEM. Comparisons were performed against the paired time points (*p < 0.001, **p < 0.01, ***p < 0.05 compared with their respective control, either LIC or D + A at their respective point times). LIC low induction control (DMEM alone), D + A DMEM plus ATP, D + S DMEM plus 10 % FCS (positive control), PTH PTH 10−9 M (positive control)