Fig. 1.

P2Y2R is a target gene of HIF-1α in human primary renal tubular cells. a HIF-1α and RNA polymerase II (RNApol II) ChIP-seq signals at the P2Y2R gene locus on chromosome 11 in MCF-7 cells indicate binding of both factors to a region approximately 23 kb upstream of the transcriptional start site of P2Y2R. HIF-1α was stabilized by 1 mM DMOG. b FAIRE assays indicate the presence of open chromatin at this site compared to a putative non-regulatory site in hPTECs. n = 3 experiments from independent hPTEC cultures. c ChIP experiments using antibodies targeting HIF-1α, HIF-1ß, and RNApol II in hPTECs reveal binding of HIF-1 to the regulatory region and interaction with the transcriptional machinery. HIF-1α was stabilized by 1 mM DMOG. n = 3 experiments from independent hPTEC cultures. d Expression qPCR for P2Y2R in lysates from hPTECs exposed to vehicle or 1 mM DMOG for 16 h. n = 13 experiments from independent hPTEC cultures. Asterisk indicates comparison to control (Ctrl)