Fig. 4.

Analysis of the expression levels of OsWRKY80 in transgenic rice lines under normal conditions. Total RNA was prepared from leaves of 4-week-old wild-type (WT) and T2 homozygous progeny of OsWRKY80-overexpressing and RNAi transgenic rice seedlings. a The expression of OsWRKY80 in different overexpression lines was analyzed by RNA gel blot. A 10 μg aliquot of total RNA was loaded per lane. The ethidium bromide stain of rRNA is shown for assessment of equal loading. b The expression of OsWRKY80 in different RNAi lines was determined by qRT-PCR analysis. The mRNA levels of OsWRKY80 were calculated relative to those of rice Actin1 (AK071586). Data are represented as mean values ± standard error (SE) for three replicates. ** indicates a significant difference at P < 0.01 between the transgenic and wild-type plants according to Duncan’s multiple range test