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. 2016 Nov 24;60:10.3402/fnr.v60.32963. doi: 10.3402/fnr.v60.32963

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© 2016 Asmaa M. Zahran et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.

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Fig. 2 — Flow cytometric detection of regulatory T cells. (a) Forward and side scatter histogram was used to define the lymphocytes population (R1). (b) The expression of CD8⁺ (R2) and CD4⁺ (R3) was assessed in lymphocytes population. (c) The expression of CD25 in CD4⁺ was detected and compared with the negative isotype control (not shown), and different gates were drowned to define CD4⁺CD25^+low cells (R5) and CD4⁺CD25^+High cells (R6). (d) The percentage of CD4⁺CD25^+high FOXP3⁺cells (CD4⁺ regulatory T cells) was determined. (e, f) The detection of CD8⁺ regulatory T cells in CD8⁺ cells. The expression of CD25 in CD8⁺ was detected. Then, CD8⁺CD25^+low cells (R8), and CD8⁺CD25^+High (R9) cells and CD8⁺CD25^+high FOXP3⁺cells (CD8⁺ regulatory T cells) were determined. (g, h) The expression of FOXP3⁺ in CD4⁺CD25^+High cells CD8⁺CD25^+high cells.