Figure 2.

Exogenous SFRP4 decreases 17-beta-estradiol (E₂) production in rat granulosa cells (GCs). GCs were cultured and treated with FSH (50 ng/ml) and testosterone (0.1 nM) in the presence or absence of rhSFRP-4 (0.5 ng/ml) for 48 hr. A. E₂ levels and B. P₄ levels were determined in conditioned media by ELISA and data were expressed relative to untreated cells (control) as pg/mg or ng/mg protein, respectively. Bars represent mean±SEM of four independent experiments assayed in triplicate. Superscript letters indicate statistically significant differences between treatment groups: *p<0.05.