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. 2016 Oct-Dec;8(4):159–168.

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Copyright© 2016 Avicenna Research Institute

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Figure 3. — rhSFRP-4 modulates P₄ biosynthesis. Granulosa cells from immature rats were cultured, hormonally stimulated, and treated sequentially with FSH for 48 hr (first 48 hr) and LH for another 48 hr (second 48 hr). A. rhSFRP-4 (0.5 or 50 ng/ml) was added alone, B. cells were simultaneously treated with FSH or LH in the presence of rhSFRP-4 (0.5 or 50 ng/ml), and C. cells were pre-treated with rhSFRP-4 3 hr before FSH treatment and then further stimulated with LH. P₄ levels were measured using ELISA and data were expressed as ng/mg protein. Bars are the mean±SEM of three independent experiments assayed in triplicate. Superscript letters indicate statistically significant differences between treatment groups: a compared to untreated cells; b compared to FSH-primed cells (***p<0.001; *p<0.05).