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Pre-treating granulosa cells (GCs) with exogenous sFRP4 prior to LH treatment abolished LH-induced P4 production. rhSFRP-4 (0.5 ng/ml) was added to GCs prior to treatment with gonadotropins or simultaneously with FSH. Conditioned media were harvested after 3, 6, 12, 24, and 48 hr, and P4 levels were measured using ELISA. Data are expressed as ng/mg protein. Data are the mean±SD from three independent experiments assayed in triplicate (***p<0.001, compared with other conditions).
