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. 2016 Oct-Dec;8(4):159–168.

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Copyright© 2016 Avicenna Research Institute

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Figure 7. — Exogenous SFRP4 differentially modulates PKB/AKT activity in rat granulosa cells (GCs) stimulated with FSH or LH. GCs were treated or pre-treated with rhSFRP-4 (0.5 ng/ml) combined with gonadotropin stimulation. A) The western blot shows a representative result of three independent experiments. B) Quantification was performed using AlphaEaseFC software for densitometric analysis of two independent experiments. β-actin levels were used as an internal control. Data are reported as the mean±SD of the fold change relative to untreated cells (set with the arbitrary value of 1.0) (*p<0.05, compared with untreated cells).