Figure 2.

Restricted amplified fragments by Mnl1 enzyme. MnlI enzyme can digest the PCR products in at least 12 hr at the temperature of 37°C and this enzymatic activity can be stopped by incubation for 20 min at a temperature of 65°C. Variant -3402T, or allele CYP2C19*17 was resistant to MnlI digestion, therefore 528 bp fragment remained intact and it did not break, while the CYP2C19*1 allele was broken into two pieces of 246 and 282 bp. The digested products were separated on 2% agarose gel and the components were visible by Syber Green. 1 indicates the 100 bp DNA ladder; 2 is a wild type genotype (CC); 3 is a heterozygote CT and 4 is TT genotype; 5 is a negative control.