Table 1.
Gene | Primer (5′–3′) | Annealing temperature (°C) | Accession number |
---|---|---|---|
c-jun | F: GGATCAAGGCGGAGAGGAA | 69 | NC_018912.2 |
R: TCCAGCCGGGCGATT | |||
c-fos | F: TCACCCGCAGACTCCTTCTC | 71.5 | NC_018925.2 |
R: GTGGGAATGAAGTTGGCACTG | |||
p53 | F: AAGAAACCACTGGATGGAGAA | 69 | NC_018928.2 |
R: CAGCTCTCGGAACATCTCGAAA | |||
β-actin | F: AGGCTGTGCTGTCCCTGTAT | 70 | NC_018928.2 |
R: ACCCAAGAAGGAAGGCTGGA | |||
GADPH | F: ACCCACTCCTCCACCTTTGAC | 72 | NC_018923.2 |
R: GTCCACCACCCTGTTGCTGTA |
Abbreviations: F forward primer, R reverse primer