Table 2.
List of analytical methods
| Biochemical parameter | Sample type | Method | Manufacturer |
|---|---|---|---|
| NEFAs | Serum | NEFA-C reagent | Wako Chemicals GmbH (Neuss, Germany) |
| Cholesterol | Serum | CHOP-PAP-method | Olympus System Reagents (OSR) -Olympus Diagnostica GmbH |
| Triglycerides | Serum | GPO-PAP method | OSR |
| Urea | Serum | GLDH method | OSR |
| Creatinine | Serum | Jaffé method | OSR |
| Total protein | Serum | Biuret method | OSR |
| Creatine kinase (CK) | Serum | IFCC method | OSR |
| Alanine aminotransferase (ALT) | Serum | IFCC method, without pyridoxal phospate | OSR |
| Alkaline phosphatase (ALP) | Serum | IFCC method | OSR |
| Haptoglobin | Serum | Phase Haptoglobin kit (colorimetric assay | Tridelta Ltd (County Kildare, Ireland) |
| C-reactive protein (CRP)a | Serum | Immunoturbidimetric method | OSR (#6147) |
| Pig-MAP | Serum | ELISA | PigChamp ProEuropa (Segovia, Spain) |
| Cortisol | Serum | ELISA | DRG Diagnostics (Marburg, Germany) |
| Cortisol | Saliva | ELISA | DRG Diagnostics |
| Glutathione peroxidase (GPx) | Erythrocyte lysate | Cumene Hydroperoxide (Ransel) | Randox Laboratories Ltd |
| Superoxide dismutase (SOD) | Erythrocyte lysate | Xanthine oxidase (Ransod) | Randox Laboratories Ltd |
| Total glutathione (tGSH) | Deproteinized blood | Glutathione Assay | Northwest (Vancouver, WA) |
aValidated for porcine samples [42]