Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 26;8:129. doi: 10.1186/s13148-016-0291-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 2 — DNA methylation regulated expression of SOX21 and SOX21-AS1 in oral cancer cells. a Schema of the locations of the SOX21 and SOX21-AS1 genes from using the UCSC Genome Browser of the human genome GRCh37/hg19 (upper panel) and mouse genome GRCm38/mm10 (lower panel). Green blocks indicate the upstream CpG islands. b SOX21 and SOX21-AS1 expression was obtained from the NGS data. c SAS and CAL27 cells were treated with 10 μM 5-Aza-dC for 4 days, and 0.25 μM TSA was added on the third day (upper panel). After the cells were treated with 5-Aza-dC and TSA, SOX21 and SOX21-AS1 expression was examined using real-time PCR (lower panel). d Schema of the map of the CpG-rich region of SOX21-AS1. CpG sites are indicated with vertical ticks, and BstU1 or TaqI restriction sites are indicated with vertical arrows, in which the arrowheads indicate the primers used for analyzing the methylation status of CpG islands (CpG1 248 bp, CpG2 266 bp, and CpG3 276 bp), obtained through COBRA in this study (upper panel). DNA methylation status of CpG islands in SAS and CAL27 cells were examined using the COBRA assay. Arrows indicate the unmethylated (u)/methylated (m) alleles (lower panel)