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. 2016 Nov 25;17:45. doi: 10.1186/s12865-016-0183-7

Fig. 2.

Fig. 2

ZA inhibits Treg cells chemotactic migration. Treg cells (5 × 10 4) were pretreated with 0, 50 or 100 μM ZA for 4 h, and placed in the upper chambers. Migration of Treg cells into the lower chambers containing DMEM with 2% FBS or CM from MDA-MB-231 cells after 2 h was analyzed. The chemotaxis index shown compares migration with the response of control cells to DMEM with 2% FBS. Values are means ± SEM of results from three independent experiments in duplicate. *P < 0.05, ** p < 0.01, ***p < 0.001