Fig. 5.

Effects of ZA on the ability of Treg cells to enhance MDA-MB-231 cell migration. a Representative pictures of migration (wound closing) of MDA-MB-231 cells grown as a mono-culture or co-culture with Treg cells at 0 and 12 h after wounding (100× magnification). b Quantification of the migration distance as a percentage of the control (MDA-MB-231 cells only). c The relative RANKL mRNA expression was evaluated by qRT-PCR from Treg cells treated with 0, 50, or 100 μM ZA for 6 h. The results from three independent experiments are shown. *P < 0.05, **p < 0.01