Table 1.

Primer sequences used for quantitative RT-PCR for mRNA expression

Target gene	Oligonucleotide sequences (5′-3′)
	Forward	Reverse
Foxp3	TGA CCA AGG CTT CAT CTG TG	GAG GAA CTC TGG GAA TGT GC
CCR4	CTT CAG TCA CCT GGC TGT CA	CTC AGC TGA ACC TGG CTA CC
TGF-β	GGA AAC CCA CAA CGA AAT C	GCT CTG ATG TGT TGA AGA AC
RANKL	ACC AGC ATC AAA ATC CCA AG	CCC CAA AGT ATG TTG CAT CC
CTLA-4	CTC AGC TGA ACC TGG CTA CC	CCA CTT GCA GAC ACC ATT TG
PD-1	ATC AAA GAG AGC CTG CGG G	GGT GGG CTG TGG GCA CT
GAPDH	TGA ACG GGA AGC TCA CTG G	TCC ACC ACC CTG TTG CTG TA