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. 2016 Nov 26;14:76. doi: 10.1186/s12951-016-0229-2

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Screening and characterization of high affinity ZNP-binding peptide. a Amino acid sequences at the indicated positions (P1–P4) of peptides bound to ZNP were determined by mass spectrometry. b Relative affinities of selected peptides labeled with FITC were assessed by measuring fluorescent intensity of ZNP-peptide complexes. Error bars mean ± SD. c Detection of the interaction of a ZBP, FPYDGGDA, with ZNP by isothermal titration calorimetry (ITC)