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. 2016 Nov 26;14:76. doi: 10.1186/s12951-016-0229-2

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Induction of ScaA specific immune responses. a Antibody responses observed in the immunized mice. Mice were immunized with ZNP, ZBP-ScaA, ZNP/ScaA, NP/ZBP-ScaA, or Alum/ZBP-ScaA (n = 3/group) twice and the levels of anti-ScaA IgG₁ and IgG_2C in the sera were measured by ELISA at 1 week after immunization. Antibody titer was assessed up to 102,400. b IFN-γ-positive CD4⁺ or CD8⁺ T cells in splenocytes stimulated with ScaA antigen were detected at 1 week after second immunization as in a. Representative dot blots obtained by FACS analysis were presented. c Average percentile of three independent experiments for IFN-γ-positive CD4⁺ or CD8⁺ T cells in splenocytes from immunized mice were presented. Error bars mean ± SD