Table 1.

Primary clock related optimized antibody dilutions and incubation times

Antibody	Dilution factor	Concentration (μg/ml)	Incubation period
CLOCK	1.1000	1.0	1 hr at RT
BMAL1	1.200	5	ON at 4°C
CRY1	1.1000	1	ON at 4°C
CRY2	1.100	2.5	ON at 4°C
PER1	1.500	2	1 hr at RT
PER2	1.500	1.1	1 hr at RT
SIRT1	1.2000	0.55	1 hr at RT

RT: Room Temperature; ON: Overnight