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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Dev Biol. 2016 Oct 5;420(1):100–109. doi: 10.1016/j.ydbio.2016.10.004

Figure 5. Altering the GLI3R/GLI3FL ratio by overexpressing Gli3A in the dorsolateral otocyst perturbs DV polarity of the otocyst.

Figure 5

(A) Reconstruction of serial section of chick inner ears collected 5-6 days after Gli3A overexpression (as shown in Fig. 3A). Frontal (top single panel) and lateral (bottom paired panels) are shown. n=5 embryos sectioned; n=1 embryo reconstructed. (B-E) ISH of sections of chick otocysts collected 12-16 hours after overexpression of Gfp alone or Gli3A and Gfp showing Pax2, Otx2, Hmx3, and Dlx5 expression. For Otx2: n=3 for both control and experimental embryos; for Pax2, Hmx3, and Dlx5: n=4 for both control and experimental embryos. Boxes show regions enlarged below, with borders of selected cells outlined. Arrowheads in B indicate dorsal otocyst cells ectopically expressing Pax2.